Standard Operating Procedures (SOPs)
SOPs contribute to development of robust methodologies to assign and locate genetic variation for traits, and facilitate comparison of experimental data from different sources
Mycological laboratory procedures: Culturing and inoculation techniques
- Media preparation (for the culture of L. maculans, P. brassicae and other fungi)
- Pyrenopeziza brassicae conidiospore production
- Bulk production of P. brassicae conidiospore inoculum
- Protocol for the production of mature apothecia of P. brassicae (for the production of ascospores)
- P. brassicae ascospore shower
- Leptosphaeria maculans inoculation methods
- L. maculans ascospore production
- L. maculans ascospore shower
Mycological laboratory procedures: transformation
Field procedures: Assessing field experiments
Seed protein and oil content and composition
- Procedure for extraction and separation of seed proteins
- Summary procedure for extraction and separation of seed proteins
- Summary procedure for NMR analysis of oil content
